Pathogenesis of Acanthamoeba keratitis: carbohydrate-mediated host-parasite interactions.

نویسندگان

  • Z Yang
  • Z Cao
  • N Panjwani
چکیده

Acanthamoeba keratitis is a sight-threatening corneal infection. In a recent study, the saccharide mannose has been shown to inhibit the binding of Acanthamoeba organisms to the epithelium of the cornea (L. D. Morton, G. L. McLaughlin, and H. E. Whiteley, Infect. Immun. 59:3819-3822, 1991). In an attempt to determine the molecular mechanism by which acanthamoebae adhere to the surface of the cornea, the present study was designed to determine whether Acanthamoeba castellanii derived from an infected human cornea (i) binds to mannose-containing glycoproteins (mannose-GPs) of corneal epithelium and (ii) expresses one or more mannose-binding proteins. Mannose-GPs of primary cell cultures of rabbit corneal epithelium were isolated by using three different agarose-conjugated, mannose-specific lectins. By electrophoresis blot-overlay assays, 35S-labeled acanthamoebae were shown to bind to mannose-GPs of corneal epithelium and to a neoglycoprotein, mannose-bovine serum albumin (mannose-BSA). 35S-labeled acanthamoebae also bound to microtiter wells coated with mannose-BSA in a concentration-dependent manner. The binding of amoebae to mannose-GPs was blocked by free methyl-alpha-D-mannopyranoside. The parasites did not bind to galactose-BSA or to many other proteins lacking mannose residues. A membrane-associated mannose-binding protein (136 kDa) of A. castellanii was isolated by affinity chromatography of detergent extracts of unlabeled parasites and of cell surface biotin-labeled parasites on a p-aminophenyl alpha-D-mannopyranoside-agarose column. The affinity-purified protein of the amoeba was shown to bind specifically to mannose-BSA. In summary, a mannose-binding protein is present on the surface membranes of Acanthamoeba, and corneal epithelial cells express Acanthamoeba-reactive GPs. One of the mechanisms of Acanthamoeba adhesion to the corneal surface may involve interactions between the mannose-binding protein of Acanthamoeba and mannose-GPs on the surface of corneal epithelium.

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عنوان ژورنال:
  • Infection and immunity

دوره 65 2  شماره 

صفحات  -

تاریخ انتشار 1997